Platelet Phosphatides: Their Separation, Identification, and Clotting Activity1

Platelet phosphatides: their separation, identification, and clotting activity.

Platelet phosphatides. Their fatty acid and aldehyde composition and activity in different clotting systems.

Previous studies from this laboratory revealed that phosphatidyl serine (PS) isolated from plate-let phospholipid extracts by silicic-acid column chromatography could replace blood platelets in tests of thromboplastin generation and prothrom-bin consumption of platelet-poor "native" plasma (1, 2). When rigorous criteria of identification were employed, it was found that phosphatidyl ethanolamin...

Platelets and platelet phosphatides in uremia.

By Gsaw n ALTSCHULER, AARON J. MARcus AND H uims L. Uu i N I HE HEMORRHAGIC tendency which develops in uremia is a well recognized clinical entity but the pathogenesis of this complication is not understood.’ In the past defective blood coagulation2 as well as increased vascular fragility3 have been suggested. The possibility of a functional blood platelet abnormality was first noted by Lewis, ...

Platelets and Platelet Phosphatides in Uremia

By Gsaw n ALTSCHULER, AARON J. MARcus AND H uims L. Uu i N I HE HEMORRHAGIC tendency which develops in uremia is a well recognized clinical entity but the pathogenesis of this complication is not understood.’ In the past defective blood coagulation2 as well as increased vascular fragility3 have been suggested. The possibility of a functional blood platelet abnormality was first noted by Lewis, ...

The paper electrophoretic separation of phosphatides.

A number of chromatographic methods have been previously reported for the separation of phosphatides. Column chromatography has been used successfully by Thannhauser and Setz (l), Klenk (a), and Hanahan et al. (3). Paper chromatographic techniques for phosphatide analysis have been reported by Bevan et al. (4), Hecht and Mink (5), Hack (6)) and Lea et al. (7). Garvin (8) has reported a separati...